Anti-allergic effect of kefir from goat milk with Lactiplantibacillus plantarum Dad-13: In Vitro evaluation

Abstract

Kefir is a type of fermented milk produced by a unique and complex mixture of bacteria and yeasts viable in a symbiotic relationship. Anti-allergic effect of goat milk kefir fermented with Lactiplantibacillus plantarum subsp. plantarum Dad-13 isolated from “Dadih” (curd made from buffalo milk from West Sumatera, Indonesia) was evaluated using rat basophilic leukemia cell line RBL-2H3 cells by identifying β-hexosaminidase release, intracellular calcium ion concentration ([Ca²⁺]i), microtubule formation and intracellular signal transduction. The results showed that goat milk kefir significantly inhibits degranulation of RBL-2H3 cells at 20 mg/mL without cytotoxicity by inhibiting the microtubule formation and the elevation in ([Ca²⁺]i ) stimulated by antigens. Immunoblot analysis demonstrated that goat milk kefir significantly downregulated phosphorylation of Syk, Akt and PI3K in the signaling pathways activated by antigen-mediated stimulation. These results suggested that goat milk kefir fermented with Lactiplantibacillus plantarum subsp. plantarum Dad-13 has the potential to serve as a functional food with anti-allergic properties.