Nobiletin prevents TMAO-induced vascular oxidative stress in rats

Abstract

Choline-rich foods in the diet are metabolized by intestinal microflora to trimethylamine (TMA). TMA is subsequently oxidized in the liver by hepatic flavin monooxygenases (FMO), yielding trimethylamine oxide (TMAO) in vivo. TMAO has been reported to cause blood vessel inflammation and induce vascular diseases and atherosclerosis. To investigate the preventive effect of nobiletin on choline chloride—induced vascular oxidative stress and inflammation, the choline chloride-treated rats were intragastric administrated of nobiletin. The serum content of IL-1β, MCP-1, IL-6 and TNF-α were analyzed by Enzyme-Linked Immunosorbent Assay (ELISA), and the contents of glutathione (GSH), malondialdehyde (MDA), nitric oxide (NO) and superoxide dismutase (SOD) were measured. The results revealed that nobiletin decreased the oxidative stress of the aorta and serum inflammatory cytokines level of the experimental rats to reduce the level of vascular inflammation.