Promoting effect of Se-allylselenocysteine on 7,12-dimethylbenz[a]anthracene (DMBA)/12-O-tetradecanoylphorbol-13-acetate (TPA)-induced skin tumorigenesis

  • An-Chin Cheng
  • Wan-Ru Jiang
  • Yu-Hsuan Hsiao
  • Vladimir Badmaev
  • Chi-Tang Ho
  • Roch-Chui Yu
  • Min-Hsiung Pan


Se-allylselenocysteine (ASC), an analogue of garlic compound, has been shown to inhibit mammary carcinogenesis in vivo and cell growth in vitro. However, the function of ASC on anti-inflammatory effects remains largely unknown. Therefore, we investigated whether ASC has an anti-inflammatory effect on lipopolysaccharide (LPS) -induced inflammation or an anti-tumour effect promoting on DMBA/TPA-induced skin tumorigenesis and tried to elucidate the mechanisms involved. Herein, the results showed that ASC inhibited LPS-induced production of nitric oxide (NO) with a decreased protein level of inducible nitric oxide synthase (iNOS) in RAW 264.7 cells. However, ASC enhanced LPS-induced cyclooxygenase-2 (COX-2) protein levels and mRNA expression. Interestingly, we found for the first time that topical application of ASC on the dorsal skin of DMBA-initiated and TPA-promoted mice significantly accelerated skin tumorigenesis and raised tumour multiplicity as compared to the positive control group (DMBA/TPA). The number of tumours that were 1–3 mm, 3–5 mm, and >5 mm in size per mouse increased in a dose-dependent manner in the ASC pre-treated groups. Pre-treatment with ASC showed a significant increase in the expression of COX-2 compared with the positive control group. In summary, that ASC may modulate the COX-2 protein expression and promoting DMBA/TPA-induced skin cancer in mice.


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How to Cite
Cheng, A.-C., Jiang, W.-R., Hsiao, Y.-H., Badmaev, V., Ho, C.-T., Yu, R.-C., & Pan, M.-H. (2020). Promoting effect of Se-allylselenocysteine on 7,12-dimethylbenz[a]anthracene (DMBA)/12-O-tetradecanoylphorbol-13-acetate (TPA)-induced skin tumorigenesis. Journal of Food Bioactives, 9.
Original Research