Figure 1. Chromatograms for 100 μg/L DMB in blank olive oil (a), blank olive oil (b), Olive oil sample N2 (c).
| Journal of Food Bioactives, ISSN 2637-8752 print, 2637-8779 online |
| Journal website www.isnff-jfb.com |
Original Research
Volume 30, June 2025, pages 40-46
Quantification of 3,3-dimethyl-1-butanol (DMB) in olive oil: a rapid and novel method
Figure
Tables
| Concentration level | |||
|---|---|---|---|
| Concentration (µg/L) | 100 | 1,000 | 5,000 |
| Recovery (%) | 100.9 | 101.6 | 99.6 |
| RSD (%) | 6.4 | 4.1 | 1.8 |
| Paramater | Data | Data |
|---|---|---|
| The method’s precision was determined by repeating the measurement six times at three concentration levels (100, 1,000, and 5,000 µg/L) prepared to assess the method’s performances for determining DMB. The average RSD values were 6.4, 4.0, and 1.8%, respectively. On the other hand, the recoveries of each level were determined as 100.9, 101.6, and 99.6 % for the same concentration levels. As expected, the lower concentration levels’ RSD and recovery levels were higher than the high-concentrated ones. | ||
| Linear regression equation | y = x.11.462.10−6 | y = x.17.860.10−6 |
| Linearity range (μg/L) | 100–5,000 | 10–100 |
| R2 | 0.9992 | 0.9983 |
| Recovery AVG (%) | 101.63 (at 1,000 μg/L) | 100.86 (at 100 μg/L) |
| Recovery SD (%) | 0.63 (at 1,000 μg/L) | 3.82 (at 100 μg/L) |
| LOD (μg/L) | 1.91 | |
| LOQ (μg/L) | 9.55 | |
| Uexpanded % | ±8.22 | ±7.0 |
| Sample code | Amount of DMB (μg/L) | U Expanded Uncertainty k = 2 (μg/L) |
|---|---|---|
| *N1 (Plus health blue multivarietal), N2 (Plus health DMB multivarietal), N3 (Fyllikon first harvest organic), N4 (Plus health green multivarietal), N5 (Agourelaio early harvest organic), N6 (unknown), N7 (Armonia monovarietal organic), N8 (Edremit type olive oil from Kırkağaç, Manisa), N9 (Market product in Türkiye). | ||
| N1 | 9.7 | 0.68 |
| N2 | 11.4 | 0.80 |
| N3 | 8.3 | 0.58 |
| N4 | 7.8 | 0.55 |
| N5 | 6.8 | 0.55 |
| N6 | 4.8 | 0.33 |
| N7 | 4.8 | 0.34 |
| N8 | 8.8 | 0.50 |
| N9 | 1.5 | 0.11 |