Journal of Food Bioactives, ISSN 2637-8752 print, 2637-8779 online
Journal website www.isnff-jfb.com

Original Article

Volume 2, Number , June 2018, pages 112-118


Antioxidant activity of faba bean extract and fractions thereof

Figures

Figure 1.
Figure 1.

UV spectra of a faba bean acetonic extract and its Fractions. 1, crude extract; 2, Fraction I; and 3, Fraction II).

Figure 2.
Figure 2.

Antioxidant activity of a faba bean acetonic extract and its fractions in a β-carotene-linoleate model system.

Figure 3.
Figure 3.

Reducing power of a faba bean acetonic extract and its fractions.

Figure 4.
Figure 4.

Scavenging effect of a faba bean acetonic extract and its fractions on DPPH radical.

Figure 5.
Figure 5.

HPLC chromatogram of a faba bean acetonic extract.

Tables

Table 1. Total phenolics, tannins content, total antioxidant activity in faba bean extract and its fractions and UV spectral data
 
Analyzed materialTotal phenolics (mg/g)Tannins (Absorbance500/g)Total antioxidant activity (mmol trolox eq./g)λmax (nm)
Crude extract56 ± 35.96 ± 0.250.88 ± 0.04276
Fraction I33 ± 23.92 ± 0.180.66 ± 0.03276
Fraction II285 ± 14116 ± 354.21 ± 0.21276

 

Table 2. Phenolic compounds identified by HPLC-DAD and HPLC-ESI-MS in a faba bean acetonic extract
 
Peak/compound numberλmax (nm)[M–H] (m/z)Ion fragments (m/z)CompoundsContent (μg/g)
1260169Gallic acid26.9 ± 2.3
2255153Protocatechuic acid18.3 ± 1.1
3280, 311137Protocatechuic aldehyde6.4 ± 0.7
4255137577, 169p-Hydroxybenzoic acid4.7 ± 0.5
5277745Gallate procyanidin dimer689 ± 33
6277203Tryptophan12.4 ± 1.8
7279289Catechin9.4 ± 0.9
8265, 348447Kaempferol hexose18.8 ±1.3
9278289Gallate procyanidin89.8±5.4
10278289, 169Gallate procyanidin18.3 ± 1.1
11275457Epigallocatechin gallate18.3 ± 1.1
12274442169Catechin gallate203 ± 1.3
13274442169Epicatechin gallate363 ± 16
14314163p-Coumaric acid25.8 ± 31
15276, 308151Vanillin3.1 ± 0.8
16268, 345510447Acetylated luteolin hexose56.8 ± 6.3
17278169Gallate procyanidin30.8 ± 1.5
18268, 335593269Apigenin hexose32.9 ± 2.6
19265, 348447285Kaempferol hexose19.9 ± 1.0
20278289, 169Gallate procyanidin28.6 ± 1.5
21265, 338489447Acetylated kaempferol hexose76.8 ± 4.6