J Food Bioact

Journal of Food Bioactives, ISSN 2637-8752 print, 2637-8779 online

Journal website www.isnff-jfb.com

Original Article

Volume 2, Number , June 2018, pages 112-118

Antioxidant activity of faba bean extract and fractions thereof

Figures

Figure 1.
UV spectra of a faba bean acetonic extract and its Fractions. 1, crude extract; 2, Fraction I; and 3, Fraction II).

Figure 2.
Antioxidant activity of a faba bean acetonic extract and its fractions in a β-carotene-linoleate model system.

Figure 3.
Reducing power of a faba bean acetonic extract and its fractions.

Figure 4.
Scavenging effect of a faba bean acetonic extract and its fractions on DPPH radical.

Figure 5.
HPLC chromatogram of a faba bean acetonic extract.

Tables

**Table 1.** Total phenolics, tannins content, total antioxidant activity in faba bean extract and its fractions and UV spectral data
Analyzed material	Total phenolics (mg/g)	Tannins (Absorbance₅₀₀/g)	Total antioxidant activity (mmol trolox eq./g)	λ_max (nm)
Crude extract	56 ± 3	5.96 ± 0.25	0.88 ± 0.04	276
Fraction I	33 ± 2	3.92 ± 0.18	0.66 ± 0.03	276
Fraction II	285 ± 14	116 ± 35	4.21 ± 0.21	276

**Table 2.** Phenolic compounds identified by HPLC-DAD and HPLC-ESI-MS in a faba bean acetonic extract
Peak/compound number	λ_max (nm)	[M–H]⁻ (m/z)	Ion fragments (m/z)	Compounds	Content (μg/g)
1	260	169		Gallic acid	26.9 ± 2.3
2	255	153		Protocatechuic acid	18.3 ± 1.1
3	280, 311	137		Protocatechuic aldehyde	6.4 ± 0.7
4	255	137	577, 169	p-Hydroxybenzoic acid	4.7 ± 0.5
5	277	745		Gallate procyanidin dimer	689 ± 33
6	277	203		Tryptophan	12.4 ± 1.8
7	279	289		Catechin	9.4 ± 0.9
8	265, 348	447		Kaempferol hexose	18.8 ±1.3
9	278		289	Gallate procyanidin	89.8±5.4
10	278		289, 169	Gallate procyanidin	18.3 ± 1.1
11	275	457		Epigallocatechin gallate	18.3 ± 1.1
12	274	442	169	Catechin gallate	203 ± 1.3
13	274	442	169	Epicatechin gallate	363 ± 16
14	314	163		p-Coumaric acid	25.8 ± 31
15	276, 308	151		Vanillin	3.1 ± 0.8
16	268, 345	510	447	Acetylated luteolin hexose	56.8 ± 6.3
17	278		169	Gallate procyanidin	30.8 ± 1.5
18	268, 335	593	269	Apigenin hexose	32.9 ± 2.6
19	265, 348	447	285	Kaempferol hexose	19.9 ± 1.0
20	278		289, 169	Gallate procyanidin	28.6 ± 1.5
21	265, 338	489	447	Acetylated kaempferol hexose	76.8 ± 4.6