Journal of Food Bioactives, ISSN 2637-8752 print, 2637-8779 online |
Journal website www.isnff-jfb.com |
Original Article
Volume 2, Number , June 2018, pages 112-118
Antioxidant activity of faba bean extract and fractions thereof
Figures
UV spectra of a faba bean acetonic extract and its Fractions. 1, crude extract; 2, Fraction I; and 3, Fraction II).
Antioxidant activity of a faba bean acetonic extract and its fractions in a β-carotene-linoleate model system.
Reducing power of a faba bean acetonic extract and its fractions.
Scavenging effect of a faba bean acetonic extract and its fractions on DPPH radical.
HPLC chromatogram of a faba bean acetonic extract.
Tables
Analyzed material | Total phenolics (mg/g) | Tannins (Absorbance500/g) | Total antioxidant activity (mmol trolox eq./g) | λmax (nm) |
---|---|---|---|---|
Crude extract | 56 ± 3 | 5.96 ± 0.25 | 0.88 ± 0.04 | 276 |
Fraction I | 33 ± 2 | 3.92 ± 0.18 | 0.66 ± 0.03 | 276 |
Fraction II | 285 ± 14 | 116 ± 35 | 4.21 ± 0.21 | 276 |
Peak/compound number | λmax (nm) | [M–H]− (m/z) | Ion fragments (m/z) | Compounds | Content (μg/g) |
---|---|---|---|---|---|
1 | 260 | 169 | Gallic acid | 26.9 ± 2.3 | |
2 | 255 | 153 | Protocatechuic acid | 18.3 ± 1.1 | |
3 | 280, 311 | 137 | Protocatechuic aldehyde | 6.4 ± 0.7 | |
4 | 255 | 137 | 577, 169 | p-Hydroxybenzoic acid | 4.7 ± 0.5 |
5 | 277 | 745 | Gallate procyanidin dimer | 689 ± 33 | |
6 | 277 | 203 | Tryptophan | 12.4 ± 1.8 | |
7 | 279 | 289 | Catechin | 9.4 ± 0.9 | |
8 | 265, 348 | 447 | Kaempferol hexose | 18.8 ±1.3 | |
9 | 278 | 289 | Gallate procyanidin | 89.8±5.4 | |
10 | 278 | 289, 169 | Gallate procyanidin | 18.3 ± 1.1 | |
11 | 275 | 457 | Epigallocatechin gallate | 18.3 ± 1.1 | |
12 | 274 | 442 | 169 | Catechin gallate | 203 ± 1.3 |
13 | 274 | 442 | 169 | Epicatechin gallate | 363 ± 16 |
14 | 314 | 163 | p-Coumaric acid | 25.8 ± 31 | |
15 | 276, 308 | 151 | Vanillin | 3.1 ± 0.8 | |
16 | 268, 345 | 510 | 447 | Acetylated luteolin hexose | 56.8 ± 6.3 |
17 | 278 | 169 | Gallate procyanidin | 30.8 ± 1.5 | |
18 | 268, 335 | 593 | 269 | Apigenin hexose | 32.9 ± 2.6 |
19 | 265, 348 | 447 | 285 | Kaempferol hexose | 19.9 ± 1.0 |
20 | 278 | 289, 169 | Gallate procyanidin | 28.6 ± 1.5 | |
21 | 265, 338 | 489 | 447 | Acetylated kaempferol hexose | 76.8 ± 4.6 |