Journal of Food Bioactives, ISSN 2637-8752 print, 2637-8779 online
Journal website www.isnff-jfb.com

Original Research

Volume 9, March 2020, pages 52-57

Phenethylamine in hot water extract of Chlorella pyrenoidosa expands lifespan of SOD1 mutant adults of Drosophila 
melanogaster at very low dose

Figures

Figure 1.
Figure 1. Effect of different concentration of WEC (a) and its Sep-Pak C18 fractions (200 μg/g) (b) on survival rate of Sod1 mutant flies.
Figure 2.
Figure 2. Size exclusion chromatography (SEC) elution pattern of compounds in Sep-Pak C18 30% acetonitrile fraction (a) and effect of SEC fractions (I–V) (200 μg/g) on survival rate of Sod1 mutant flies (b).
Figure 3.
Figure 3. Precursor ion scan of AccQ derivatives in SEC fraction III and IV. *Shows reagent peak.
Figure 4.
Figure 4. Effect of different concentration of phenethylamine (a), isopentylamine (b) and pentylamine (c) on survival rate of Sod1 mutant flies.

Table

Table 1. Recovery of solid phase extraction fractions using Sep-Pac C18 cartilage
 
FractionDry weight (g)Recovery to WEC (%)
unabsorbed10.368.67
10% acetonitrile0.64
30% acetonitrile0.593.9
AAH0.060.404