@article{Guo_Zhang_Zhang_Wang_Ren_Jia_Liu_Zhang_2022, title={Transport mechanism of eurycomanone from Eurycoma longifolia Jack across Caco-2 cells model}, volume={17}, url={http://www.isnff-jfb.com/index.php/JFB/article/view/276}, DOI={10.31665/JFB.2022.17301}, abstractNote={<p><em>Eurycomanone </em>is the main active ingredient of <em>Eurycoma longifolia </em>Jack with anti-cancer, anti-malaria, improving male sexual dysfunction and other effects.The poor lipid solubility of e<em>urycomanone</em> is potential reason for its low bioavailability. However, the transmembrane absorption mechanism has not been reported. In this study, the Caco-2 cell monolayer model was used to investigate the influence of different&nbsp;factors&nbsp;on&nbsp;the eurycomanone transmembrane absorption, including time, concentration, temperature, P-glycoprotein inhibitors (verapamil or cyclosporin A) and collateral transport enhancer (sodium desoxycholate or EDTA).The results showed that the apparent permeability coefficient (Papp) of eurycomanone was lower than 10<sup>-6 </sup>cm/s with poor oral absorption. The Papp was not dependent on concentrations or temperatures. The addition of paracellular transport enhancer promoted the absorption of eurycomanone(<em>P</em> &lt; 0.05), whereas p-glycoprotein inhibitors had no effect.Taken together, this study indicated that absorption of eurycomanone&nbsp; may undergo passive transmembrane transport and paracellular transport.</p&gt;}, journal={Journal of Food Bioactives}, author={Guo, Wu-Yan and Zhang, Huan and Zhang, Yue-Yang and Wang, Shu-Yan and Ren, Jian and Jia, Yong-Qing and Liu, Rui and Zhang, Bo}, year={2022}, month={Mar.} }